23 10 月

Inhibitory effect and action mechanism of citral against black rot in pitaya fruit

2024 年 10 月 23 日
栽培管理技術, 期刊論文
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作者： Luo, Donglan ; Ye, Shengjie ; Qu, Guangfan ; Ba, Liangjie
刊名： Physiological and molecular plant pathology
期刊所屬國別：
出版年： 202405
關鍵字： Black rot
摘要： "Blackspot, caused by Alternaria alternata, is a significant postharvest disease in pitaya. Currently, there are limited effective fungicides to manage this disease. This study aimed to evaluate the antifungal activity of citral against the blackspot pathogen and to determine the protective effect of citral on pitaya. Our results demonstrated that citral effectively curtailed the mycelial growth and spore germination of Alternaria alternata, with a minimum inhibitory concentration (MIC) of 0.6 μL/mL. Observations under a light microscope revealed that citral-treated hyphae displayed characteristics such as sparseness, roughness, twisting, shriveling, and irregular internode separation, along with prominent vacuolation. Propidium iodide and 2,7-Dichlorodi-hydrofluorescein diacetate staining results revealed that the mycelia from the MIC citral-treated group emitted robust fluorescence, with the average fluorescence intensities being 4.03 and 8.09 times higher than the control group, respectively. Furthermore, citral treatment resulted in a decline in the lipid content and dry weight of the hyphae, increased nucleic acid and protein leakage, enhanced exosporial conductivity, and augmented superoxide dismutase (SOD) and catalase (CAT) enzyme activities. Additionally, it fostered the accumulation of hydrogen peroxide (H2O2) and malondialdehyde (MDA). Fruit damage inoculation assays indicated that an appropriate concentration of citral (1/2 MIC, MIC) could notably diminish the fruit spot diameter expansion. Thus, citral shows promise as a potential alternative fungicide for managing postharvest black rot in pitaya. •Citral could significantly inhibit mycelial growth and spore germination of Alternaria alternata.•Citral treatment destroyed the cell membrane integrity of Alternaria alternata, which could significantly increase the amount of hyphal material released.•Citral treatment increased H2O2 and MDA accumulation and antioxidant enzyme activity of Alternaria alternata.•Citral treatment decreased lesion development on pitaya fruit black rot."
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