23 10 月

Bacillus siamensis N-1 improves fruit quality and disease resistance by regulating ROS homeostasis and defense enzyme activities in pitaya

2024 年 10 月 23 日
採前處理, 栽培管理技術, 期刊論文
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作者： Xu, Jian ; Zheng, Yaru ; Peng, Dengfan ; Shao, Yuanzhi ; Li, Rui ; Li, Wen
刊名： Scientia horticulturae
期刊所屬國別：
出版年： 202404
關鍵字： Disease resistance ; Natural immunity
摘要： "•N-1 treatment significantly decreased disease incidence and lesion area in pitaya fruit.•N-1 treatment increased contents of ASA, TSS and TA, but decreased those of MDA, H2O2 and O2−.•Disease incidence negatively correlated with activities of PAL, GLU and CHI.•Disease incidence positively correlated with H2O2 and O2−contents.•Lesion area negatively correlated with HuSOD, HuCAT and HuAPX expressions. Pitaya is one of the most important tropical fruit, but its postharvest disease is quite serious. Bacillus siamensis N-1 obtained in our previous report is an antagonistic strain against Fusarium oxysporum, which often causes soft rot of pitaya. The purpose of this study is to clarify the biocontrol effect of N-1 on soft rot in pitaya, results showed that N-1 treated fruit showed significantly enhanced disease resistance with lower disease incidence and lesion area. Compared to control, N-1 treatment effectively increased fruit firmness, contents of ascorbic acid (AsA), total soluble solids (TSS) and titratable acid (TA), but significantly reduced respiration rate, weight loss, hydrogen peroxide (H2O2), superoxide anion (O2−) and malonaldehyde (MDA) content. Moreover, N-1 treatment significantly increased activities of phenylalanine deaminase (PAL), β-1,3-glucanase (GLU), chitinase (CHI), peroxidase (POD) and polyphenol oxidase (PPO), and also changed transcriptions of the corresponding enzyme genes. Correlation analysis further showed that fruit disease incidence was significantly negatively correlated with activities of PAL, GLU, CHI, but positively correlated with respiration rate and contents of H2O2 and O2−. Additionally, expressions of HuSOD, HuCAT and HuAPX are significantly negatively correlated with lesion area, indicating that they played critical roles in regulating reactive oxygen species (ROS) homeostasis in postharvest pitaya. Taken together, N-1 treatment could activate the disease-resistant enzyme system and avoid excessive accumulation of ROS, thereby maintaining fruit quality and reducing postharvest disease."
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