番石榴病害綜合管理模式建立及監測技術之開發(第2年/全程3年)

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計畫名稱: 番石榴病害綜合管理模式建立及監測技術之開發(第2年/全程3年)
計畫主持人: 周浩平
共同計畫主持人:
計畫編號: 100農科-9.2.2-高-K3
計畫主管機構: 行政院農業委員會
計畫執行機構: 行政院農業委員會高雄區農業改良場
全程計畫年: 2011
關鍵字: 放線菌;Streptomyces spp.;根瘤線蟲;Meloidogyne spp.;萬壽菊;African Marigold;番石榴立枯病;Guava Myxosporium Wilt;恆溫環形核酸增幅技術;Loop-mediated Isothermal Amplification
摘要: 本計畫擬建立放線菌與萬壽菊等忌避作物栽培防治番石榴根瘤線蟲標準操作模式並評估實際防治效果、並針對番石榴立枯病菌、果腐病等病原進行殺菌劑篩選。應用已分離之放線菌Streptomyces coliecolor(K269)、Streptomyces lydicus (K285)、以及Streptomyces saraceticus(K305、K364、K400)等菌株配合蝦蟹殼粉及農業廢棄物配方,並導入萬壽菊栽培,評估對根瘤線蟲之實際防治效果。此外了解轄區農民栽培及病蟲害防治習性,除篩選有效之藥劑可應用於整枝修剪時之傷口保護,亦開發恆溫環形核酸增幅技術(Loop-mediated Isothermal Amplification, LAMP)等較靈敏之監測技術,於整枝修剪時定期進行立枯病之監測,正確掌握防治時機,最後建立ㄧ套完整之番石榴健康管理模式並可於田間實際應用。This study aimed to assess the applicability of Streptomyces spp. and African marigold in controlling Meloidogyne spp. on guava. Different Streptomyces strains, including Streptomyces coliecolor (K269), Streptomyces lydicus (K285), and Streptomyces saraceticus (K305, K364, and K400), were mixed with synergist/diluent (composed of barley, rice husk, oyster shell powder, shrimp and crab shell powder, and corn powder) to make soil amendments and also cultured with African marigold in the field. On the other hand, to survey of the cutural and diseases controlling habits from farmers that we can select effective pestcides could be used to control the guava Myxosporium wilt on pruning-wounds and establish a sensitive detection technique just like loop-mediated isothermal amplification to monitor the populations of guava Myxosporium wilt pathogen, then we can set up the most appropraite timing to control the disease. Establishment of the model of guava health management will be the most importment work, ultimately.
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