|作者： 官曉青；岳慶熙；蔡智賢；李堂察；Kuan, Hsiao-ching；Yueh, Ching-shi；Tsay, Jyh-shyan；Lee, Tan-cha
|關鍵字： 非洲菊；碳源；培植體；乳糖；種原保存；Gerbera；Carbon source；Explants；Lactose；Germplasm preservation
|摘要： 本研究探討醣種類及濃度對非洲菊幼苗Gerbera jamesonii在試管內生長之影響及種原保存上的應用。以非洲菊幼苗爲培殖體，於1/2MSH培養基中添加不同 醣類作爲碳源，以添加1%乳糖有效降低培殖體的生長速率；延長繼代培養的時間達5個月，之後培殖體移到3%蔗糖的培養基中可恢復生長活力。建議使用1%乳糖作爲非洲菊種原保存的碳源。Experiments were conducted to study the influence of carbon sources and concentrations on the in vitro growth and maintenance of Gerbera jamesonii plantlets for the purpose of efficient mother stock or gerplasm preservation. Cluster of shoots production from young capitulum explants of Gerbera were successfully cultured on a basal medium (1/2 MSH). The result of carbon sources experiment indicated that in order to prolong the time interval between subcultures of the in vitro preserved Gerbera plantlets, the addition of lactose to the medium was effective in lowering the growth rate of explants. The optimum lactose concentration was 1%. The interval between successive subculture could be extended to as long as 5 months. The growth vigor could be resumed after transferring the explants to a culture medium containing 3% sucrose.